anti il 2 jes6 1a12 bioxcell  (Bio X Cell)

Journal: iScience

Article Title: Imaging of biphasic signalosomes constructed by checkpoint receptor 2B4 in conventional and chimeric antigen receptor-T cells

doi: 10.1016/j.isci.2024.111669

Figure Lengend Snippet:

Article Snippet: IL-2 purified (JES6-1A12) , e-Bioscience , 14-7022-85; RRID: AB_468406.

Techniques: Control, Purification, Virus, Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Labeling, Retroviral, Plasmid Preparation, Luciferase, Software

Journal: Frontiers in Immunology

Article Title: Activation of the aryl hydrocarbon receptor inhibits neuropilin-1 upregulation on IL-2-responding CD4 + T cells

doi: 10.3389/fimmu.2023.1193535

Figure Lengend Snippet: Conditions to differentiate into CD4 + T cell subsets.

Article Snippet: Autocrine production of IL-2 was neutralized by the addition of an anti-IL2 antibody ( InVivo MAb anti-mouse IL-2; clone JES6-1a12; BioXcell; Cat # BE0043-1) at 10 ng/ml, 100 ng/ml and 1000 ng/ml.

Techniques: Concentration Assay

Journal: Frontiers in Immunology

Article Title: Activation of the aryl hydrocarbon receptor inhibits neuropilin-1 upregulation on IL-2-responding CD4 + T cells

doi: 10.3389/fimmu.2023.1193535

Figure Lengend Snippet: The reduction of CD25 + Nrp1 + expression resulting from AhR activation is dependent on IL-2. (A) CD4 + T cells isolated from NOD mice splenocytes were co-cultured with 1000 ng/ml, 100 ng/ml, 10 ng/ml or 0 ng/ml of anti-IL-2. All cells were also co-cultured with IL-12, anti-IL4, and CD3/CD28 Dynabeads. AhR was activated with 100 nM Cl-BBQ. Following incubation at 37°C for 4 days, cells were stained, and Nrp1 expression was analyzed on CD4 + CD25 + Foxp3 - cells. A two-way ANOVA was performed. * indicates p-value <0.05. ** indicates p-value <0.01. n=4 mice/condition. (B) CFSE-labeled cells from B6 mice were injected into F1 hosts and treated i.p. with anti-IL-2 or isotype control and 15μg/kg TCDD or vehicle. Nrp1 expression on proliferating donor (CFSE dilute) CD4 + CD25 + cells were analyzed on day 2.A one-way ANOVA was performed. **** indicates p-value ≤0.0001 n=5 mice/group. Each data point represents an individual mouse.

Article Snippet: Autocrine production of IL-2 was neutralized by the addition of an anti-IL2 antibody ( InVivo MAb anti-mouse IL-2; clone JES6-1a12; BioXcell; Cat # BE0043-1) at 10 ng/ml, 100 ng/ml and 1000 ng/ml.

Techniques: Expressing, Activation Assay, Isolation, Cell Culture, Incubation, Staining, Labeling, Injection, Control